Web Content Delivery Optimization

Milliseconds matters, when they’re counted. If we consider the life of the universe into one single year, then on 31 December at 11:59:59.5 PM, “speed” was transportation’s concern, and now after 500 milliseconds it is web’s, and no one knows whose concern it would be in coming milliseconds, but at this very moment; this thesis proposes an optimization method, mainly for content delivery on slow connections. The method utilizes a proxy as a middle box to fetch the content; requested by a client, from a single or multiple web servers, and bundles all of the fetched image content types that fits into the bundling policy; inside a JavaScript file in Base64 format. This optimization method reduces the number of HTTP requests between the client and multiple web servers as a result of its proposed bundling solution, and at the same time optimizes the HTTP compression efficiency as a result of its proposed method of aggregative textual content compression. Page loading time results of the test web pages; which were specially designed and developed to capture the optimum benefits of the proposed method; proved up to 81% faster page loading time for all connection types. However, other tests in non-optimal situations such as webpages which use “Lazy Loading” techniques, showed just 35% to 50% benefits, that is only achievable on 2G and 3G connections (0.2 Mbps – 15 Mbps downlink) and not faster connections

Effects of Acidified Yeast and Whey Powder on Performance, Organ Weights, Intestinal Microflora, and Gut Morphology of Male Broilers

ABSTRACT In the present study, we investigated the effects of acidified dried whey and acidified yeast product and their combination on broiler growth characteristics, intestinal microflora and gut morphology. Seven hundred and fifty day-old Ross 308 male broilers were fed one of 10 dietary treatments with 5 replicates in a randomized design. A corn-soybean meal based diet (control group) was supplemented with 3 levels (0.15, 0.30 and 0.45%) of acidified whey powder (AW), acidified yeast product (AY), and acidified whey powder and yeast product (AWY). Acidified yeast product improved ADG and FCR at d 42, but the effects of additives on productive performance were not significant across the entire rearing period. The highest level of AY increased the population of Lactobacillus spp., decreased that of E. coli and did not affect the bacterial total count at d 42. Birds fed 0.45% AY showed higher villus height and better gut morphology compared to control birds. In conclusion, acidified yeast product increased the population of Lactobacillus, inhibited the growth of E. coli and improved gut morphology and structure, but did not affect growth performance and relative organ weight

A Clinical and Molecular Genetic Study of 50 Families with Autosomal Recessive Parkinsonism Revealed Known and Novel Gene Mutations

In this study, the role of known Parkinson�s disease (PD) genes was examined in families with autosomal recessive (AR) parkinsonism to assist with the differential diagnosis of PD. Some families without mutations in known genes were also subject to whole genome sequencing with the objective to identify novel parkinsonism-related genes. Families were selected from 4000 clinical files of patients with PD or parkinsonism. AR inheritance pattern, consanguinity, and a minimum of two affected individuals per family were used as inclusion criteria. For disease gene/mutation identification, multiplex ligation-dependent probe amplification, quantitative PCR, linkage, and Sanger and whole genome sequencing assays were carried out. A total of 116 patients (50 families) were examined. Fifty-four patients (46.55; 22 families) were found to carry pathogenic mutations in known genes while a novel gene, not previously associated with parkinsonism, was found mutated in a single family (2 patients). Pathogenic mutations, including missense, nonsense, frameshift, and exon rearrangements, were found in Parkin, PINK1, DJ-1, SYNJ1, and VAC14 genes. In conclusion, variable phenotypic expressivity was seen across all families. © 2017, Springer Science+Business Media New York

A Clinical and Molecular Genetic Study of 50 Families with Autosomal Recessive Parkinsonism Revealed Known and Novel Gene Mutations

In this study, the role of known Parkinson�s disease (PD) genes was examined in families with autosomal recessive (AR) parkinsonism to assist with the differential diagnosis of PD. Some families without mutations in known genes were also subject to whole genome sequencing with the objective to identify novel parkinsonism-related genes. Families were selected from 4000 clinical files of patients with PD or parkinsonism. AR inheritance pattern, consanguinity, and a minimum of two affected individuals per family were used as inclusion criteria. For disease gene/mutation identification, multiplex ligation-dependent probe amplification, quantitative PCR, linkage, and Sanger and whole genome sequencing assays were carried out. A total of 116 patients (50 families) were examined. Fifty-four patients (46.55; 22 families) were found to carry pathogenic mutations in known genes while a novel gene, not previously associated with parkinsonism, was found mutated in a single family (2 patients). Pathogenic mutations, including missense, nonsense, frameshift, and exon rearrangements, were found in Parkin, PINK1, DJ-1, SYNJ1, and VAC14 genes. In conclusion, variable phenotypic expressivity was seen across all families. © 2017, Springer Science+Business Media New York